LIBRO + ANEXOS NEUMOLOGÍA PEDIÁTRICA

Libro Blanco de las ACES Pediátricas 2024 212 ❚ J. Cellular and humoral immunity 1. Normal function a. Know that the secretory IgA in the upper airways is responsible for complement- independent neutralization of respiratory viruses b. Know that there are IgG subclasses c. Know that IgG is the major immunoglobulin within the alveoli d. Know that the major immunoglobulin within the upper airway is IgA e. Know the role of lysozyme in hydrolyzing structural components of bacterial cell walls f. Know that IgG subclass deficiency can predispose to infection, even when total IgG concentration is normal g. Know the role of neutrophils in the defense of the lung against bacterial, viral, mycobacterial, fungal, and parasitic diseases h. Know the role of lymphocytes in the defense of the lung against bacterial, viral, mycobacterial, fungal, and parasitic diseases i. Know the role of eosinophils in the defense of the lung against bacterial, viral, mycobacterial, fungal, and parasitic diseases 2. Immunodeficiency states a. Understand the immunodeficiency states associated with pulmonary infection b. Know that recurrent infection may be the result of a defect in phagocytosis (chronic granulomatosis disease) c. Know that IgA deficiency is associated with atopy and airway reactivity d. Identify the immune deficiency state existing after lung trans- plantation and the organisms most likely to be pathogenic in that setting e. Know the manifestations of post-transplant lymphoproliferative disorder and that it is caused by Epstein-Barr virus (EBV) f. Know the roles of graft-versus-host disease and infection in the development of chronic lung disease after bone marrow transplantation 8. Cell Biology and Biochemistry A. Cell and molecular biology 1. Cell kinetics a. Know that the S (synthesis) stage is the period of DNA replication and the period of "labelling" with markers of proliferation (eg, tritiated thymidine, BrdU, PCNA) 2. Cell differentiation a. Know the characteristics that distinguish progenitor cells from differentiated cells 3. Protein synthesis a. Understand the relationships between DNA, mRNA, and amino acid sequences b. Recognize that protein expression may be modified at the levels of transcription, translation, and post-translation c. Understand the general principles of mRNA detection by Northern hybridization, microarray in situ hybridization, and reverse transcriptase polymerase chain reaction (RT PCR) d. Understand the general principles of protein detection by Western blot, immunoblot, 2-D gel electrophoresis, and immunohistochemical analyses e. Know that protein synthesis occurs in free ribosomes and in the rough endoplasmic reticulum